Fig. 6.

PIK3CA^H1047R is compatible with monolayer definitive endoderm differentiation. (A) Schematic of the protocol for definitive endoderm differentiation in monolayer culture. (B) Real-time quantitative PCR analysis of lineage marker expression during differentiation in the presence of DMSO (control) or the p110α-specific inhibitor BYL719 at 100 nM. Data from two independent experiments (EXP) with WT (WT/WT) vs. PIK3CA^{H1047R/H1047R} (H1047R/H1047R) are shown side by side (60- and 84-h time points were only assessed once). Two cultures of each of two clones per genotype were profiled. The time course data for PIK3CA^WT/H1047R (WT/H1047R) vs. WT cells are from a single experiment using two cultures of one clone per genotype. Gene expression was scaled internally to the mean value of an appropriate time point, and resulting values are arbitrary. B, baseline (0 h). See also SI Appendix, Fig. S7.