Fig. 4.
Mito-specific Interactions between the NTE of RRFmt and the 39S mitoribosomal subunit proteins. (A) The N-terminal region of L27 (purple) in the human 55S–RRFmt complex has shifted by ∼9 Å toward the peptidyl tRNA-binding site compared with the positions of L27 in the porcine (gray) (25) (PDB ID: 5AJ4) and empty human (green) (24) (PDB ID: 3J9M) mitoribosomes, to interact with the NTE (red) of RRFmt. In this conformation, L27 would block the binding of tRNA in the 39S P site. Mito-specific interactions between the NTE and mitoribosomal proteins are shown in panels (B) L27 and (C) L16 (dark cyan). Thumbnails to the left depict overall orientations of the 55S mitoribosome, with semitransparent 28S (yellow) and 39S (blue) subunits, and overlaid positions of ligands. Landmarks on the thumbnails are same as in Fig. 2.