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. 2019 Apr 8;116(17):8283–8288. doi: 10.1073/pnas.1815675116

Fig. 5.

Fig. 5.

Mito-specific interactions between the NTE of RRFmt and the 16S rRNA components of the 39S subunit. (A) The NTE (red) of RRFmt interacts with several functionally important segments of the peptidyl-transferase center (PTC), including the P loop (blue), H89 (yellow), H90 (pink), and A loop (H92) (green). (B) Mito-specific interactions of the NTE with the nucleotide bases of H89, H90, and the A loop. Color codes are same as in A. Thumbnails on the top depict overall orientations of the 55S mitoribosome in A and B, with semitransparent 28S (yellow) and 39S (blue) subunits, and overlaid positions of RRFmt. Landmarks on the thumbnails are same as in Fig. 2. (C) Overall location of the NTE on the 39S subunit (semitransparent blue), as seen from the subunit’s interface side, with the rRNA components of PTC that extend up to the GTPase-associated center (GAC), which includes the α-sarcin-ricin stem loop (H95). Both PTC and GAC are depicted as dashed circles. (D) Secondary structure of the PTC region of the 16S rRNA, highlighting helices that are color-coded as in A–C, and interacting nucleotides with red ovals.