Table 2.

Test for correlation between tumor volume and differential biomarker level

Patient	Total volume of adenomas	Prepolypectomy value for patient minus median of tumor-free controls
		F5	ITIH4	LRG1	VTN	CRP	PI16
1	88	0.2006	0.1682	−0.4543	0.6935	0.4216	−0.2411
2	83	0.2705	0.2666	−0.1245	0.0220	−0.2630	0.2372
3	45	0.3427	0.6316	2.1361	1.6167	5.6626	−0.1137
7	900	0.3438	0.6752	−0.3025	0.9683	0.5870	−0.2807
9	19	0.3245	0.4132	0.3231	0.6464	0.4393	−0.1040
10	266	0.0067	−0.3177	−0.6473	0.1241	0.2493	−0.2497
15	111	0.0346	0.1481	0.3792	−0.0883	0.5677	0.4204
17	69	−0.0431	−0.0961	2.4822	1.2550	1.0925	0.0398
20	100	0.1949	0.3348	−0.5072	0.6225	−0.0682	0.0118
24	211	0.2733	−0.1468	0.7395	0.1380	0.5134	−0.0742
32	231	0.6716	0.7877	0.3385	−0.1937	−0.1975	−0.1876
33	38	−0.0376	−0.2199	−0.4052	−0.7240	−0.1866	−0.0825
Spearman	ρ	0.24	0.06	−0.25	−0.15	−0.04	−0.38
	Two-sided P value	0.42	0.83	0.40	0.63	0.89	0.21
Pearson	r	0.26	0.32	−0.27	0.13	−0.12	−0.41
	Two-sided P value	0.42	0.32	0.40	0.68	0.70	0.18

The total volume of the colonic adenomas carried by the patients shown in SI Appendix, Table S1 was calculated from their CTC images. The corresponding levels of each of the conserved markers (F5, ITI4, LRG1, and VTN) and the two rapidly reversible markers (CRP and PI16) were determined, relative to the labeled standard probe of each marker. A Spearman test was carried out for correlation between the monotonic rank orders of tumor volume and biomarker level. The Pearson r and Spearman ρ values and their P values are consistent with the null hypothesis—a lack of correlation between tumor volume and the level of each informative serum biomarker.