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. 2019 Apr 8;11(4):1307–1319. doi: 10.1093/gbe/evz075

Fig. 1.

Fig. 1.

—Cytogenetic analysis of representatives of the Gelechiid and Scythridid assemblages. Chromosomes were counterstained with DAPI (blue); female derived genomic probes (A–D) were labeled by Cy3 (red). (A and B) GISH in Tuta absoluta (Gelechiidae, Gelechiid assembl.): (A) female mitotic metaphase consisting of 2n = 58 elements; note that the W chromosome is one of the two largest chromosomes in the complement; (B) female pachytene nucleus; the probe labeled the W chromosome in the WZ bivalent and chromosome ends of most bivalents. (C and D) GISH in Sitotroga cerealella (Gelechiidae, Gelechiid assembl.): (C) female mitotic metaphase consisting of 2n = 60 chromosomes; the W chromosome is not conspicuously larger than the other chromosomes; note DAPI-stained small rod-shaped bodies, probably corresponding to bacteria; (D) late pachytene female nucleus; the probe identified the W chromosome in the WZ bivalent. (E and F) Mitotic complements of Coleophora laricella (Coleophoridae, Scythridid assembl.) stained with DAPI: (E) male mitotic metaphase consisting of 2n = 58 chromosomes; note a pair of large chromosomes (arrowheads); (F) female mitotic metaphase comprising 2n = 58 chromosomes; note a pair of large chromosomes (arrowheads). Bar = 10 µm.