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. 2019 Jan 3;24(1):138–152.e8. doi: 10.1016/j.stem.2018.12.001

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Dpf2 Deletion Impairs ESC Differentiation

(A) GO analysis for biological processes associated with genes differentially expressed upon Dpf2 deletion in ESCs.

(B) qPCR analysis for transcript levels of the indicated lineage-specific genes on days 4 and 7 of EB differentiation of Dpf2^fl/fl and Dpf2^−/− cells.

(C) Global gene expression profiles of Dpf2^fl/fl and Dpf2^−/− EBs. Neuro, neural ectoderm markers; Meso, mesoderm markers; Endo, endoderm markers; Pluri, pluripotency-associated genes.

(D) Images of H&E-stained teratomas from Dpf2^fl/fl ESCs. Magnification, 630×.

(E) As in (D), except for Dpf2^−/− ESCs. Magnification, 200×.

(F) qPCR analysis for transcript levels of the indicated lineage-specific genes in day 6 EBs from Dpf2^fl/fl, Dpf2^−/−, and Dpf2^−/− with ectopically (exo) expressed Tbx3.