Figure 5.

Regulation of Meso-endoderm Differentiation by Dpf2 and Eed via Tbx3

(A) Clustering of Dpf2 binding events in ESCs and day 2 and 4 EBs. The genome was divided into 500-bp bins and a bin called bound (blue) or unbound (white) based on the presence of a DPF2 peak.

(B) GO analysis for enriched biological process for genes associated with DPF2 peaks from different clusters defined in (A), within ± 20 kb of the TSS.

(C) Transcript levels of the indicated endoderm markers in day 4 EBs from Dpf2^fl/fl, Eed^−/−, and Eed/Dpf2 double KO determined by qPCR.

(D) As in (C), except for pluripotency-associated genes in Dpf2^fl/fl, Dpf2^−/−, Eed^−/−, and Dpf2 and Eed double KO ESCs.

(E) As in (C), except for Tbx3 in day 4 EBs from Dpf2^fl/fl, Eed^−/−, and Eed/Dpf2 double KO ESCs.

(F) As in (C), except for various endoderm markers in 4-day EBs induced from Eed^−/− and Eed^−/−/Tbx3 kd ESCs.

(G) As in (C), except for various mesoderm markers and Tbx3 in day 7 EBs from Dpf2^fl/fl, Eed^−/−, and Eed/Dpf2 double KO ESCs.

(H) As in (C), except for various neuroectoderm markers in day 6 EBs from Dpf2^fl/fl, Dpf2^−/−, and Eed/Dpf2 double KO ESCs.