Figure 7.

Model for the Regulation of ESC Differentiation by Dpf2 and Eed via the Control of Tbx3 Expression

Dpf2 and Eed regulate Tbx3 expression via modulation of the H3K27ac level at the DE and the H3K27me3 level at the IE. Loss of Dpf2 induces downregulation of Tbx3 via a decrease of both H3K27ac and pluripotency TF binding on the DE, leading to impaired differentiation into meso-endoderm. In Eed^−/− ESCs, H3K27me3 levels decrease at the IE, whereas the binding of pluripotency TFs increases, resulting in Tbx3 upregulation and enhanced differentiation to meso- and endoderm. In Dpf2^−/−/Eed^−/− ESCs, Tbx3 expression is restored to physiological ESC levels, as is the potential for differentiation into endo- and mesoderm.