Figure 2. Development of cardiac-specific GCN5L1 knockout (cKO) mice.
(A) Mice with LoxP sites surrounding exon 3 of GCN5L1 were crossed to αMHC-MerCreMer mice to produce tamoxifen-inducible, cardiomyocyte-specific knockouts of GCN5L1 (GCN5L1 cKO). (B, C) MerCreMer expression in GCN5L1FL/FL cardiomyocytes leads to significant reductions in GCN5L1 transcript and protein expression. (D, E) No overt contractile phenotype is observed in the isolated hearts of cardiac-specific inducible knockouts. N= 5-12. **** = P < 0.0001 vs. control.