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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Transl Res. 2019 Jan 7;207:70–82. doi: 10.1016/j.trsl.2019.01.001

Figure 2.

Figure 2.

Flow chart depiction of e-cig aerosol production, exposure, and elimination. (A) The software interface (SI) controlled voltage delivered to the programmable atomizer (PA), which produced the e-cig vapor plume, as well as puff duration and frequency. The puffs traveled to the custom-engineered inhalation chamber (IC) and then through an activated charcoal filter (FL), which eliminated harmful emissions from the IC exhaust. (B) Maternal Vaping Paradigm: Pregnant dams underwent vaping for three hours, five days a week, from gestational day (GD) 5–19. A one second puff was dispensed every 20 seconds. (C) Maternal and pup Vaping Paradigm: Pregnant dams underwent vaping for 2 hours, five days a week, from GD 5–21. Dams gave birth on GD 22, and dams and pups underwent vaping treatment from postnatal day (PND) 4–10, for 2 hours a day. A one second puff was dispensed every 20 seconds.