Figure 1. Cancer and stromal cells express fascin protein in the ovarian cancer tumor microenvironment.
a-c. Immunohistochemical analysis of fascin expression in formalin-fixed paraffin embedded tissues. a. Representative images of fascin expression in normal omentum and omental metastases. S, stroma. T, tumor. A, adipocytes. Arrows, tumor-stroma interface. Arrowhead, mesothelial cells. Size bar, 500 μm. b. Box plot analysis of fascin expression in the stroma or cancer compartment of all ovarian cancer tumor subtypes (n=201) and representative images of immunohistochemistry scoring for stroma or cancer compartments in tumors. Boxes indicate 25th percentile, median, and 75th percentile. *** = p<0.001 calculated from Wilcoxon signed-rank test. c-d. Kaplan-Meier analysis of overall survival (OS; n=76 patients ; c) and progression-free survival (PFS; n=74 patients; d) across non-serous ovarian cancer subtypes in patients with 0 level (negative, blue, OS n=35 patients, PFS n=33 patients) fascin expression versus 1–3 level (positive, red, OS n=41 patients, PFS n=n=41 patients) fascin expression in cancer cells of primary tumor. P-values calculated from Cox regression analysis.