Table 1.

Oligonucleotides used

Oligo name	Oligo sequence
Construction of Sso7d-Sau
Sso_F	5′-CATGCCATGGCAACAGTAAAGTTCAA-3′
Linker_R	5′-GTCGACGCTTGCTGATGAGCCTCCG-3′
PS_F	5′-CGGAGGCTCATCAGCAAGCGTTG-3′
PS_R	5′-TTTTCCTTTTGCGGCCGCTTTTGCATCATACCAAGTTGC-3′
Generation of hybrid Sau-TBD
PT1_F	5′-CATGCCATGGAACATCATCATCATCATCATTCAGCAAGCGTTGAAG-3′
PT1_R	5′-GATACCACGA ACCAGCTGCATCATGGAT-3′
PT2_F	5′-GTTGTGTTTAACCCTTCGTCTCCTAAGCAATTAGGTG − 3′
PT2_R	5′-CGCGGATCCTTATTTTGCATCATACC-3′
T BD_F	5′-TGCAGCTGGTTCGTGGTATCAGCCTAAAGG − 3′
TBD_R	5′-CACCTAATTGCTTAGGAGACGAAGGGTTAAACACAAC-3′
Amplification of TrxA
Trx_F	5′-CATGCCATGGGCAGCGATAAAATTATTCACCTG-3′
Trx_R	5′-TTTTCCTTTTGCGGCCGCCGCCAGGTTAGCGTCGAGG-3′
Primer used for polymerase activity assay
M13-40	5′-GTTTTCCCAGTCACGACG-3′
Primer used for processivity assay
M13-40 LF	5′-FAM-GTTTTCCCAGTCACGACGTTGTAAAACGACGGCC-3′
Amplifying efficiency assay
200_F	5′-AATTTGCTGAGATTAACATAGTAGTCAATG-3′
200_R	5′-ACAATGTTTTATCTTACTGTCTTTGATGAG-3′
500_F	5′-ACTACTAAATCCTGAATAGCTTTAAGAAGG-3′
500_R	5′-CAGAAAGCTAAATATGGAAAACTACAATAC-3′
600_F	5′-TGAGTATTGGTTTATTTGGCGATTATTATC-3′
600_R	5′-AAATAATTCCTGAAGATATTAAAGAGCGTC-3′
700_F	5′-CTCAAAAGGTATAGTTAAATCACTGAATCC-3′
700_R	5′-AGAAAGCTAAATATGGAAAACTACAATACG-3′
800_F	5′-TTTTGAATAATAAATGTTACTGTTCTTGCG-3′
800_R	5′-AATTATTGGAAAAGAGTTATGTATCAGTGC-3′
900_F	5′-TGAGTATTGGTTTATTTGGCGATTATTATC-3′
900_R	5′-TGCACAAAAGAAATTACCTTCATATTTAAC-3′
1000_F	5′-CCCATCGTCTTTCTGATTTAATAATAGATG-3′
1000_R	5′-CAGAGGGATCTAGAATATGATGAAAGATAG-3′