Figure 3.

Accumulation of transcripts encoding TD (pDH14.2), importin-α (pDH40.1), PIOX (pDH41.6), and the putative kinase cofactor pDH63.5 in response to biotic and abiotic stimuli. RNA was extracted from shoots 72 h after inoculation with TMV with carborundum powder, 24 h after inoculation with the disarmed strain LBA4404 of A. tumefaciens (AT), and 12 h after folivory by caterpillars (CAT), repeated wounding in 30-min intervals (WDG), application of MeJA to leaves (MeJA), exposure to ethylene (ETH), application of MSA to leaves (MSA), and of ABA to roots (ABA), respectively. The control lanes correspond to untreated plants (CON 1), plants treated with lanolin paste (CON 2), and plants treated with carborundum powder (CON 3), respectively. Equal blotting was verified by hybridization with the 18S-rRNA probe.