Nucleotide and deduced amino acid sequence of
clone pTD13 encoding full-length N. attenuata TD. The stop
codon is marked with a star, putative polyadenylation signals within
the 3′-UTR are underlined, and an in-frame nonsense codon in the 5′-UTR
is indicated by a dot. The partial cDNA clone pTD18.2 starts at
position 487 (black arrow) of the nucleotide sequence of pTD13 and
extends 46 bp beyond the 3′ end of pTD13. In pTD18.2 we found two
conservative base substitutions marked with triangles at position 1,126
(T to A) and position 1,801 (T to C), respectively. The cDNA clone
pDH14.2 generated by DDRT-PCR starts at position 1,820 (white arrow)
and matches in sequence to pTD18.2. Numbers refer to the nucleotide
sequence not counting the poly(A) stretches. The GenBank accession
number of pTD13 is AF229927.