Table I.
Summary of pDH clones containing cDNA inserts isolated by DDRT-PCR
| Clone | Length | Anchor Primer | GenBank Accession No. | BLAST Results
|
|
|---|---|---|---|---|---|
| Sequence similarity | E value | ||||
| bp | |||||
| pDH6.1 | 210 | A1 | AW191805 | Bell pepper Sn-1 gene (X79230) | 2 × 10−20 |
| pDH9.4 | 645 | A1 | AW191806 | G. coronatum β-tubulin (AAF08229) | 6 × 10−82 |
| pDH9.8 | 618 | A1 | AW191807 | No similarities found | – |
| pDH10.4 | 582 | A1 | AW191808 | Caenorhabditis elegans cosmid F54B3 (Z48583) | 7 × 10−4 |
| pDH10.7 | 571 | A1 | AW191809 | Arabidopsis unknown protein (AAC14534) | 4 × 10−32 |
| pDH11.1 | 711 | A2 | AW191810 | B. japonicum FixO protein homolog (B47468) | 7 × 10−25 |
| pDH14.2 | 289 | A2 | AW191811 | Tomato threonine deaminase gene (M61915) | 6 × 10−6 |
| pDH19.3 | 485 | A2 | AW191812 | Arabidopsis dehydration-induced protein RD22 (Q08298) | 2 × 10−22 |
| pDH20.2 | 273 | A3 | AW191813 | Potato germin-like protein mRNA (AF067731) | 9 × 10−14 |
| pDH21.4 | 347 | A3 | AW191814 | No similarities found | – |
| pDH23.5 | 252 | A3 | AW191815 | Human chromosome 4 clone B366O24 (AC004067) | 5 × 10−3 |
| pDH25.4 | 172 | A3 | AW191816 | Rice protein similar to retrotransposon RIRE1 (BAA88170) | 6 × 10−6 |
| pDH30.4 | 163 | A3 | AW191817 | No similarities found | – |
| pDH31.5 | 123 | A3 | AW191818 | No similarities found | – |
| pDH39.1 | 672 | A4 | AW191819 | Tomato cDNA clone cLEB8F13 (AI483151) | 1 × 10−138 |
| pDH40.1 | 615 | A4 | AW191820 | Tomato importin α-subunit (O22478) | 7 × 10−6 |
| pDH41.6 | 433 | A5 | AW191821 | Tobacco mRNA for pathogen-induced oxygenase (AJ007630) | 1 × 10−123 |
| pDH46.4 | 289 | A6 | AW191822 | Tobacco blp5 mRNA for luminal-binding protein BiP (X60058) | 4 × 10−53 |
| pDH48.4 | 604 | A6 | AW191823 | Tomato cDNA clone cLER17A21 (AI775929) | 1 × 10−14 |
| pDH55.2 | 164 | A6 | AW191824 | Arabidopsis ferredoxin-dependent glutamate synthase GLU2 (AAC78549) | 4 × 10−19 |
| pDH55.7 | 163 | A6 | AW191825 | Wild tobacco unknown gene upstream of cnx5 (AF124162) | 5 × 10−48 |
| pDH61.1 | 270 | A7 | AW191826 | Tomato lhbC1 gene for LHCII type III (X60275) | 8 × 10−39 |
| pDH63.5 | 374 | A8 | AW191827 | Potato mRNA for GAL83 protein (AJ012215) | 8 × 10−86 |
| pDH64.4 | 74 | A8 | AW191828 | Tetrameles nudiflora 18S ribosomal RNA gene (U41502) | 2 × 10−23 |
| pDH64.7 | 566 | A8 | AW191829 | Nicotiana sylvestris mRNA for the small subunit of Rubisco (X01722) | 1 × 10−146 |
| pDH68.1 | 152 | A9 | AW191830 | No similarities found | – |
| pDH69.3 | 162 | A9 | AW191831 | Tobacco Mg protoporphyrin IX chelatase (CHL H) mRNA (AF014052) | 8 × 10−81 |
| pDH73.2 | 276 | A10 | AW191832 | No similarities found | – |
The length of the inserts is given in base pairs excluding the 3′-poly(A) stretch. In addition, anchor primers used in combination with the single random primer R1 for DDRT-PCR and the GenBank accession nos. are listed. Sequence similarities and expectation (E) values obtained from BLAST searches correspond with the highest scoring matches with nucleotide or protein sequences found in the GenBank or The Arabidopsis Information Resource databases. Accession nos. of the heterologous sequences are given in parentheses.