Fig. 4.

Cell surface binding of 12 antibodies is specific for the odontoblastic differentiation. A Immunohistochemistry of the antigenic molecules in tooth slices. The antigenic molecules recognized by 12 mAbs expressed in odontoblastic cell rich layer or perivascular region in pulp core (arrowheads). B Odontoblastic specificity of mAbs during the differentiation process. Three stages of odontoblastic differentiation were prepared as pre-odontoblast (hDPCs), odontoblast (hDPCs treated with BMPs, Od), and mature odontoblast (dentin forming cells, Od+Additives). Cell surface binding of mAbs on these cells were analyzed by immunocytometric analysis using by FACS (a). 1, hDPCs; 2, Od; 3, Od+Additives. Quantification of antibody binding was represented as mean fluorescence (b). Statistics were analyzed by Student’s t test, and asterisk indicated the significant difference between two samples. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant