Fig. 6.

Cell sorting potentials of mAb OD40 and OD46. A The dental pulp cells are sorted by the magnetic-activated cell sorting system with anti-OD40 (a) and anti-OD46 (b) antibodies. After sorting, the antibody binding affinity was confirmed by FACS. B Relative mean fluorescence intensity was quantified by WinMDI program. 1, unsorted dental pulp cells; 2, antibody-negative cells; 3, antibody-positive cells. Cell population with significant antibody binding intensity was indicated as empty histograms. Statistics were analyzed by Student’s t test. *, P < 0.001. C Mineralization of OD40- and OD46-positive cells. Mineral deposits were stained by alizarin red (a), and were quantified (b) (see Additional file 1). Statistics were analyzed by Student’s t test, and asterisk indicated the significant difference between two samples. ****, P < 0.05