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. 2019 Apr 27;50:28. doi: 10.1186/s13567-019-0648-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Interference and overexpression of UBXN1. A, B siRNA interference fragments reduced the expression of UBXN1. The relative expression of UBXN1 was assessed by Western blotting after 24 h of interference. Control: normal group; NC1: transfection reagent group; siRNA: fragment 1. B The lentiviral plasmid overexpressed UBXN1. The plasmid was transfected into cells for 36 h, and the relative expression of UBXN1 was assessed by Western blotting. Control: normal group; NC2: transfection reagent group; Overexpression: transfection plasmid group. C Molecular modulators of UBXN1 do not affect cell viability. Cell viability was determined by an MTT assay after treatment with the interference fragment (SiRNA) or overexpression plasmid (Plenti-CMV-GFP-2A-Puro-UBXN1) for 48 h. The data are the means ± SEMs of three independent experiments (t-test, ^#p > 0.05). Control: normal group; IF1: interference fragment 1 group; TP: transfection plasmid group.