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Primers used to construct the recombinant plasmids
| Gene | Accession number | Purpose | Sequence (5′–3′) |
|---|---|---|---|
| TGEV-S1 | DQ811786.2 | Construction of pGBKT7-S1 | F: CTCTTCCAGCCCTCCTTCC |
| R: GGTCCTTGCGGATGTCG | |||
| TGEV-S1-t | XM_003353824.1 | Construction of pGEX-4T-S1 | F: CGGAATTCGAGAAGGCTCTGGCCCTCA |
| R: GGCGCATTTCGTCTTCCTGG | |||
| UBXN1-t | DQ811786.2 | Construction of pET-28a-UBXN1 | F: TCGGTCGACTCAACTGGGACA-CTTCTT |
| R: GCGGTCGACTTAGTTTGTCTAATAATA |
