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Primer sequences for real-time quantitative PCR
| Gene | Accession number | Sequence (5′–3′) | Size (bp) | Concentration |
|---|---|---|---|---|
| β-Actin | XM_003124280.2 | F: CTCTTCCAGCCCTCCTTCC | 97 | 0.5 µM |
| R: GGTCCTTGCGGATGTCG | ||||
| UBXN1 | XM_003353824.1 | F: TTGGAGCTTGTGGCCCAGAA | 139 | |
| R: GGCGCATTTCGTCTTCCTGG | ||||
| TGEV-N | NC_038861.1 | F: TTCAACCCCATAACCCTCCAACAA | 136 | |
| R: GGCCCTTCACCATGCGATAGC |
