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. 2019 Apr 27;10:126. doi: 10.1186/s13287-019-1229-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Vascular surface area (expressed in square micrometers per field) determined by immunohistochemical analysis of CD31 expression in wound sections. Vascularized area was significantly increased compared to the untreated group (Linitul) (***p < 0.001), SF group (^∆∆∆p < 0.001), or Wj-MSCs-Edge-treated group (^###p < 0.001), respectively, according to one-way ANOVA. Results are shown as mean ± SD of the most vascularized areas measured in three different mice of each group, corresponding to images made at × 400 magnification. Experimental groups: SF (wounds covered with silk fibroin scaffold), Wj-MSCs-Edge (Wj-MSCs injected at the wound edge), Wj-MSCs-SF (wounds covered with silk fibroin scaffold cellularized with Wj-MSCs), and Wj-MSCs-SF+Edge (wounds treated with Wj-MSCs injected at the wound edge and cellularized silk fibroin scaffold onto the wound bed)