Table 3.
Antibodies and dilutions used in the analyses of NDRG1 in canine tissues and cells
| Name | Catalog number | Producer | Dilutions | ||
|---|---|---|---|---|---|
| IHC | IF | WB | |||
| Monoclonal anti-NDRG1 antibody produced in mouse | WH0010397 | Sigma-Aldrich, Merck, Darmstadt, Germany | 1/2000 | 1/2000 | 1/2000 |
| Polyclonal anti-NDRG1 antibody produced in goat | PA5–18109 | Invitrogen, Thermo Fisher Scientific | 1/750 | NA | NA |
| Polyclonal anti-NDRG1 antibody produced in rabbit | HPA006881 | Sigma-Aldrich, Merck | 1/100 | 1/100 | 1/1000 |
| Monoclonal phospho-specific anti-NDRG1 (Thr346) produced in rabbit | 5482 | Cell Signaling Technology, Leiden, Netherlands | NA | 1/500 | 1/1000 |
| Monoclonal phospho-specific anti-NDRG1 (Ser330) produced in rabbit | ab124713 | Abcam, Cambridge, United Kingdom | NA | 1/75 | 1/10000 |
| Anti-glial fibrillary acidic protein | Z0334 | Dako, California, United States | 1/500 | 1/500 | NA |
| Anti-Iba1 | 019–19,741 | Fujifilm Wako Chemicals, Neuss, Germany | 1/250 | NA | NA |
IHC Immunohistochemistry, IF Immunofluorescence, WB Western blot, NA Not analyzed