Table 1.
Analytical testing of tag-based NGS
| RS | cfDNA input (ng) | Library (pM) | Median Read Cov | Median Mol Cov | EGFR gene variants | VAF (%) | LOD (%) | Allele Mol Cov | Tot Read Cov |
|---|---|---|---|---|---|---|---|---|---|
| HD780 (5%) | 30 | 430 | 53,562 | 4365 | E746_A750delELREA | 5.81 | 0.05 | 265 | 62,561 |
| V769_D770insASV | 4.17 | 0.05 | 220 | 47,935 | |||||
| T790M | 5.46 | 0.05 | 272 | 73,309 | |||||
| L858R | 5.02 | 0.1 | 119 | 56,740 | |||||
| HD780 (1%) | 30 | 408 | 27,829 | 3614 | E746_A750delELREA | 0.91 | 0.05 | 37 | 34,718 |
| V769_D770insASV | 0.85 | 0.05 | 34 | 23,487 | |||||
| T790M | 0.91 | 0.05 | 40 | 37,057 | |||||
| L858R | 1.15 | 0.1 | 25 | 29,792 | |||||
| HD780 (0.1%) | 30 | 450 | 28,269 | 3778 | E746_A750delELREA | 0.14 | 0.05 | 6 | 35,994 |
| V769_D770insASV | 0.1 | 0.05 | 4 | 23,378 | |||||
| T790M | 0.11 | 0.05 | 5 | 33,633 | |||||
| L858R | 0.09 | 0.1 | 2 | 27,703 | |||||
| HD780 (0%) | 30 | 570 | 32,314 | 2353 | E746_A750delELREA | 0 | nd | 0 | |
| V769_D770insASV | 0 | nd | 0 | ||||||
| T790M | 0 | nd | 0 | ||||||
| L858R | 0 | nd | 0 | ||||||
| HD780 (0.1%) | 20 | 705 | 22,117 | 2734 | E746_A750delELREA | 0.03 | 0.05 | 1 | 29,492 |
| V769_D770insASV | 0.1 | 0.05 | 3 | 20,616 | |||||
| T790M | 0.19 | 0.05 | 6 | 28,438 | |||||
| L858R | 0.13 | 0.1 | 2 | 19,308 |
RS Reference Standard, Median Read Cov Median Read Coverage, Median Mol Cov Median Molecular Coverage, VAF Variant Allele Frequency, LOD Limit of Detection, Allele Mol Cov Allele Molecular Coverage, Tot Read Cov Total Read Coverage, nd not detected, HD Horizon Discovery