Figure 3.

The combination therapy of CFL with 5-fluorouracil reduced the expressions of Ca²⁺ intracellular level in HT-29 cells. (A) Control group. (B) Group was treated with 5FU only. (C), (D), and (E) HT-29 cells were treated with CFL 5, 10, and 20 μg/mL, respectively, after 5-fluorouracil, (F) HT-29 cells were treated with CFL only. Green arrow: Ca²⁺ intracellular positive cell. All images were magnified at representative 4 independent experiments (G). Number of Ca²⁺ intracellular positive cells. The result of [iCa²⁺] expression also showed that combination groups have lower expression than 5FU only group, respectively (p<0.05; p<0.05; p<0.05). ^∗p-value < 0.05; ^∗∗p-value < 0.001.