Figure 2.

Transcript accumulation in rosette or stem leaves of flowering plants. Leaves (being two positions older than the youngest rosette leaf or two positions younger than the first stem-born leaf) of five replicate N. attenuata plants were continuously wounded and supplied with W or R from M. sexta larvae for 80 min, creating one row of puncture wounds every 20 min and harvesting 20 min after the final treatment. Untreated leaves were harvested as controls (C). Hybridization with an 18S rRNA probe demonstrates equal loading. The type of expression pattern (I, IIa) is indicated.