Figure 2.

Changes of lipid accumulation of renal tissue in ORG model. (a) Representative Oil Red O staining images of renal tissue in different groups (magnification ×1000). (b) The relative mRNA and protein expression levels of ADRP of the renal cortex were measured by real-time quantitative PCR and Western blot assay. The relative protein expression level was expressed as the target protein/β-actin ratio. Values are represented as mean ± SD. ^∗P < 0.05 vs. control group, ^#P < 0.05 vs. ORG model group. (c) Double immunofluorescence staining of ADRP and nephrin of the ORG model. The localization of ADRP (red spots), nephrin (green spots), and merged image (yellow spots) in the frozen section of renal tissue of the ORG model (×400) is shown as indicated.