Figure 7.

Effects of inhibiting CD36 on leptin-induced activation of NLRP3 inflammasome and IL-1β secretion of podocyte. (a) Differentiated podocytes were incubated in medium, medium containing 15 ng/mL leptin, or medium containing both 15 ng/mL leptin and 50 μM SSO, respectively. (b) Differentiated podocytes were transiently transfected with 50 pmol control siRNA or CD36 siRNA, respectively. After 24 h, cells were incubated in medium or medium containing 15 ng/mL leptin as indicated, respectively. (a) and (b) The relative mRNA and protein expression levels of NLRP3, pro-caspase-1, and IL-1β of podocytes were measured by real-time quantitative PCR and Western blot assay. Values are represented as mean ± SD (n = 3). ^∗P < 0.05 vs. control group, ^#P < 0.05 vs. leptin + SSO group (a) or leptin + CD36 siRNA group (b).