Table 1.
Primer sequences for PCR analysis in animal and cellular experiments.
| Target | Primer sequence (5′-3′) | Length (bp) |
|---|---|---|
| Nephrin | Forward GTCTGGGGACCCCTCTATGA | 209 |
| Reverse CAGGTCTTCTCCAAGGCTGT | ||
|
| ||
| Podocin | Forward CAGAAGGGGAAAAGGCTGCT | 200 |
| Reverse GATGCTCCCTTGTGCTCTGT | ||
|
| ||
| Desmin | Forward GTTTCAGACTTGACTCAGGCAG | 106 |
| Reverse TCTCGCAGGTGTAGGACTGG | ||
|
| ||
| ADRP | Forward CTGGTGAGTGGCCTGTGT TA | 199 |
| Reverse AAGCACACGCCTTGAGAG AA | ||
|
| ||
| CD36 | Forward ATGGGCTGTGATCGGAACTG | 60 |
| Reverse AGCCAGGACTGCACCAATAAC | ||
|
| ||
| SREBP-1 | Forward GCGTGGTTTCCA ACATGACC | 188 |
| Reverse TAGTGCCTCCTTTGCCACTG | ||
|
| ||
| PPARα | Forward CTGCAGAGCAACCATCCAGAT | 70 |
| Reverse GCCGAAGGTCCACCATTTT | ||
|
| ||
| NLRP3 | Forward TCTGCACCCGGACTGTAAAC | 131 |
| Reverse CATTGTTGCCCAGGTTCAGC | ||
|
| ||
| Pro-caspase1 | Forward ACAAGGCACGGGACCTATG | 237 |
| Reverse TCCCAGTCAGTCCTGGAAATG | ||
|
| ||
| IL-1β | Forward CGCAGCAGCACATCAACAAG | 118 |
| Reverse GTGCTCATGTCCTCATCCTG | ||
|
| ||
| GAPDH | Forward TGTGAACGGATTTGGCCGTA | 202 |
| Reverse GATGGGCTTCCCGTTGATGA | ||