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Activation of the SIRT3/SOD2 signaling pathway was essential to the antioxidative effects of melatonin. (a, b) The efficiency of SIRT3 siRNA was confirmed by Western blot (n = 3 per group, &&P < 0.01 vs. the scramble SiRNA group). (c–h) Western blot of SIRT3, Ac-SOD2, SOD2, cyto-c, NOQ1, and Nrf2 (n = 3 per group, ∗P < 0.05 and ∗∗P < 0.01 vs. the H2O2 group, #P < 0.05 and ##P < 0.01 vs. the H2O2 + MT group).
