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. 2019 Apr 11;2019:3698742. doi: 10.1155/2019/3698742

Table 2.

Effect of naphthalene acetic acid (NAA), on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.

Explant
MS + NAA (mg/L) Morphogenetic Response Callus
Mean fresh weight (mg) Mean dry weight (mg)
Leaf 1.0 Friable white callus 311.7 ± 0.31c 19.1 ± 0.23c
2.0 Friable white callus 313.5 ± 0.23d 19.5± 0.67b
3.0 Friable pale brown callus with roots 303.6 ± 0.55e 19.0 ± 0.54d
4.0 Friable pale brown callus with roots 291.5 ± 0.18f 17.2± 0.33e
5.0 Friable pale brown callus with roots 242.4 ± 0.23j 15.6 ± 0.80g

Stem
(Inter node)
1.0 Friable white callus 153.3 ± 0.14l 2.2± 0.43m
2.0 Friable white callus 255.4 ± 0.40i 4.9 ± 0.45l
3.0 Friable white callus 303.6 ± 0.47e 10.1 ± 0.13h
4.0 Pale brown callus 343.1 ± 0.21b 15.8 ± 0.36f
5.0 Pale brown callus 412.8 ± 0.49a 25.1 ± 0.15a

Root 1.0 White friable callus 256.5 ± 0.38i 9.5 ± 0.19k
2.0 White friable callus 277.4 ± 0.14g 16.1 ± 0.03f
3.0 Pale brown callus 262.1 ± 0.21h 14.6 ± 0.43h
4.0 Brown callus 258.4 ± 0.74i 13.9 ± 0.67i
5.0 Dark brown callus 243.8 ± 0.36j 13.2 ± 0.31i

Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.