Table 2.
Effect of naphthalene acetic acid (NAA), on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.
Explant |
MS + NAA (mg/L) | Morphogenetic Response | Callus | |
---|---|---|---|---|
Mean fresh weight (mg) | Mean dry weight (mg) | |||
Leaf | 1.0 | Friable white callus | 311.7 ± 0.31c | 19.1 ± 0.23c |
2.0 | Friable white callus | 313.5 ± 0.23d | 19.5± 0.67b | |
3.0 | Friable pale brown callus with roots | 303.6 ± 0.55e | 19.0 ± 0.54d | |
4.0 | Friable pale brown callus with roots | 291.5 ± 0.18f | 17.2± 0.33e | |
5.0 | Friable pale brown callus with roots | 242.4 ± 0.23j | 15.6 ± 0.80g | |
| ||||
Stem (Inter node) |
1.0 | Friable white callus | 153.3 ± 0.14l | 2.2± 0.43m |
2.0 | Friable white callus | 255.4 ± 0.40i | 4.9 ± 0.45l | |
3.0 | Friable white callus | 303.6 ± 0.47e | 10.1 ± 0.13h | |
4.0 | Pale brown callus | 343.1 ± 0.21b | 15.8 ± 0.36f | |
5.0 | Pale brown callus | 412.8 ± 0.49a | 25.1 ± 0.15a | |
| ||||
Root | 1.0 | White friable callus | 256.5 ± 0.38i | 9.5 ± 0.19k |
2.0 | White friable callus | 277.4 ± 0.14g | 16.1 ± 0.03f | |
3.0 | Pale brown callus | 262.1 ± 0.21h | 14.6 ± 0.43h | |
4.0 | Brown callus | 258.4 ± 0.74i | 13.9 ± 0.67i | |
5.0 | Dark brown callus | 243.8 ± 0.36j | 13.2 ± 0.31i |
Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.