Table 3.
Effect of Kinetin (Kn) on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.
| Explant | MS + Kn (mg/L) | Morphogenetic Response | Callus | |
|---|---|---|---|---|
| Mean fresh weight (mg) | Mean dry weight (mg) | |||
| Leaf | 1.0 | No response | -- | -- |
| 2.0 | Friable white callus | 201.4 ± 0.11h | 11.1 ± 0.14f | |
| 3.0 | Friable green callus | 226.3 ± 0.41f | 13.5 ± 0.22d | |
| 4.0 | Pale brown callus | 334.6 ± 1.02c | 18.5± 0.68b | |
| 5.0 | Pale brown callus | 349.5 ± 0.46a | 22.8 ± 0.19a | |
|
| ||||
| Stem (Inter node) | 1.0 | Friable white callus | 218.6 ± 0.25g | 3.6 ± 0.19i |
| 2.0 | Friable White callus | 233.3 ± 0.23f | 4.2 ± 0.17h | |
| 3.0 | Friable White callus | 264.9 ± 0.91e | 7.8 ± 0.23g | |
| 4.0 | Pale brown callus | 309.4 ± 0.87d | 11.5 ± 0.61e | |
| 5.0 | Pale brown callus | 343.2 ± 0.64b | 14.4 ± 0.26c | |
|
| ||||
| Root | 1.0 | Single shoot bud | - | - |
| 2.0 | Two shoot buds | - | - | |
| 3.0 | Multiple shoot buds | - | - | |
| 4.0 | Multiple shoot buds | - | - | |
| 5.0 | Multiple shoot buds | - | - | |
Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.