Table 4.
Effect of TDZ and BAP on shoot bud differentiation from 3 times subcultured leaf, stem, and root callusof Rauwolfia tetraphylla L.
| Three times sub-cultured callus | TDZ (mg/L) |
BAP (mg/L) |
No. of shoot buds |
|---|---|---|---|
| Leaf | 0.10 | 2.00 | 4 ± 0.12f |
| 0.25 | 2.00 | 25 ± 0.25a | |
| 0.50 | 2.00 | 10 ± 0.17d | |
| 0.75 | 2.00 | 8 ± 0.11e | |
| 1.00 | 2.00 | 2 ± 0.15g | |
|
| |||
| Stem | 0.10 | 2.00 | 4 ± 0.43f |
| 0.25 | 2.00 | 20 ± 0.76b | |
| 0.50 | 2.00 | 14 ± 0.13c | |
| 0.75 | 2.00 | 4 ± 0.28f | |
| 1.00 | 2.00 | 2 ± 0.26g | |
Data represents average of three replicates. Each replicate consists of 10 cultures. Data scored at the end of 2 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.