Fig. 2.

Production of decellularized cell-derived ECM from cultures of human chondrocytes, BMSC and SMSC. Phase contrast microscopy and fluorescent microscopy DAPI/phalloidin staining taken in different fields of view before and after the treatment with 20 mM NH₄OH solution with 0.5% Triton X-100 in PBS solution to confirm the success of the decellularization process. DAPI stains cell nuclei blue and phalloidin stains actin-rich cell cytoskeleton red. Scale bar 100 μm.