Basis of the OMA1 activity using fluorescence-based technology. A) Fluorescence is released when OMA1 recognizes and cleaves the OPA1 8-mer peptide (fluorescence reporter) presumably at the RA site. B) Experimental workflow. [Abbreviations: MCA - 7 Methoxycoumarin 4 ylacetyl; DNP - 2,4 Dinitrophenyl; TPEN - N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine.