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. 2019 Apr 26;39(4):BSR20181584. doi: 10.1042/BSR20181584

Figure 4. Infection of BV-2 cells and quantification of IκB-α level in mRNA and protein.

Figure 4

(A) After transfection for 72 h, GFP expression of Lv-Control, Lv-NC, and Lv-shRNA-IκB-α transfecting groups showed a gene delivery efficiency close to 100%. Top: dark field, bottom: corresponding fluorescence field. Gene delivery efficiency was estimated by accounting the ratio of cells expressing GFP of the total cell number. MOI = 20; magnification, 120×. (B) Quantitative analysis of the level of IκB-α in mRNA with or without transfection. (C) Representative images and quantitative analysis of the level of IκB-α in protein with or without transfection. Tests were carried out on three biological replicates, *P<0.05 versus Lv-NC; **P<0.01 versus Lv-NC.