Skip to main content
. 2019 Apr 26;39(4):BSR20182293. doi: 10.1042/BSR20182293

Figure 1. PTHrP (1–34)-induced fibronectin up-regulation is independent of TGF-β1 signaling.

Figure 1

MCs were treated with 100 nM PTHrP (1–34) for indicated time. (A) mRNA level of TGF-β1 was assessed by RT-PCR (*P<0.05 vs control, n=4). (B) Protein expression of TGF-β1 and TβRII was detected by western blot, with β-actin used as loading control (*P<0.05 vs control, n=3). (C) Protein level of fibronectin was assessed by western blot (*P<0.05 vs control, n=4). (D) MCs were pretreated with TGFβ receptor inhibitor SB431542 before PTHrP (1–34) incubation for 24 or 48 h. Protein level of fibronectin was assessed by western blot (*P<0.05 vs control, n=3).