Figure 4. PTHrP (1–34)-induced Src activation and EGFR transactivation are independent of MMPs-mediated HB-EGF release.

MCs were treated with CRM197 (A), the HB-EGF inhibitor or GM6001 (B), a pan-specific MMPs inhibitor, prior to 100 nM PTHrP (1–34) incubation. The extent of phosphorylation of Src at Y416, EGFR at Y845, and Akt at S473 was detected by Western blot (*P<0.05 vs control, n=4).