Figure 5. PTHrP (1–34)-induced fibronectin up-regulation in rat MCs is dependent on NOX and Src/EGFR/PI3K signaling.

MCs were pretreated with NOX inhibitor apocynin (A), Src inhibitor SU6656 (B), EGFR kinase inhibitor gefitinib (C), or PI3K inhibitor LY294002 (D) followed by incubation with 100 nM PTH (1–34) for 24 or 48 h. The protein level of fibronectin was assessed by western blot, with β-actin used as loading control (*P<0.05 vs control, n=5).