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. 2019 Apr 26;39(4):BSR20190477. doi: 10.1042/BSR20190477

Figure 5. LC-MS/MS detection of berberine in the mitochondria of HepG2 cells (Mean ± S.D., n=3).

Figure 5

HepG2 cells were incubated with 10 μM berberine at 37°C for 0.25, 0.5, 1, 2, 4, or 6 h, respectively. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in 0.5 ml chilled water and were broken by repeated freezing and thawing. The concentration of berberine in whole cells or mitochondria isolated according to the manufacturer’s instructions was quantified using the LC-MS/MS method. The concentration of berberine was calibrated according to protein content. * indicates P<0.05 while ** indicates P<0.01 versus mitochondria.