Fig. 3.
Cbl-b degradation may not be a general phenomenon associated with CD40 costimulation. Splenic BALB/c B cells (5 × 106) were stimulated with anti-IgM and anti-CD40 for 5, 15 and 30 min, and lysed. The cell lysates were blotted with anti-Lyn, Fyn and PI3-K (p85), respectively. The data shown is representative of three independent experiments.