Table 2. Experimental water condition for river puffer, Takifugu obscurus and tiger puffer, T. rubripes in this anaesthetic experiment*.
| Test parameters | Condition |
|---|---|
| pH | 7.6±0.02 |
| DO (dissolved oxygen; mg/L; Saturated concentration in 26°C) | 7.5±0.32 |
| Ammonia (ppm) | 0.00 |
| Nitric acid (ppm) | 1.8±0.14 |
| Nitrous acid (ppm) | 0.01 |
| Conductivity (µs/cm) | 238 |
*
Test parameters were analyzed at 1 hr before experiment. The values are means of triplicate groups. Dissolved oxygen, pH and salinity were measured using an oxygen measurement electrode and a multi-data logger system (Oxyguard, Denmark). Ammonia, nitric acid, nitrous acid and conductivity were measured using spectrophotometer (DR2800, Colorado, USA).