Table 4. Effects of different clove oil dose and water temperature on the river puffer, Takifugu obscurus and tiger puffer, T. rubripes*.
Temperature (°C) | Dose (ppm) | Time (sec) | |||||
---|---|---|---|---|---|---|---|
River puffer | Tiger puffer | ||||||
Exposure (E) | Recovery (R) | Time ratio (sec)* (R/E) | Exposure (E) | Recovery (R) | Time ratio (sec)* (R/E) | ||
20 | 15 | 164.4±6.38aA | 171.8±6.21dA | 0.84±1.01aA | 160.4±8.53aB | 167.8±8.62dA | 1.04±1.01bA |
30 | 125.9±5.72bA | 194.7±4.83cA | 0.86±1.15aA | 110.6±5.47bA | 203.3±6.33cA | 1.83±1.15aA | |
45 | 86.6±3.76cA | 208.5±4.11bA | 1.09±1.58aA | 93.0±3.07cA | 211.0±4.88bA | 2.26±1.58aA | |
60 | 79.1±1.52dA | 224.3±3.08aA | 1.36±1.63aA | 82.7±1.95cA | 219.8±3.19aA | 2.65±1.63aA | |
024 | 15 | 168.9±5.33aA | 175.8±5.72cA | 0.72±1.25aA | 182.7±6.18aA | 152.0±8.06dB | 0.83±1.30bA |
30 | 110.3±4.48bB | 184.1±4.23bB | 0.69±1.00aA | 113.4±4.58bA | 194.7±6.14cB | 1.71±1.34aA | |
45 | 83.1±3.62cA | 190.7±3.14bB | 0.67±1.20aA | 89.2±3.87cA | 208.1±4.11bA | 2.33±1.06aA | |
60 | 71.4±2.12dB | 206.6±2.51aB | 1.18±1.24aA | 77.2±1.63dB | 221.4±3.03aA | 2.86±1.88aA | |
28 | 15 | 138.5±4.01aB | 161.5±4.31cB | 1.04±0.96aA | 117.5±9.01aC | 155.5±6.73dB | 1.32±0.96bA |
30 | 90.8±3.52bC | 176.1 ±3.78cC | 0.86±0.75aA | 94.2±7.45bB | 187.6±4.10cC | 1.99±0.75bA | |
45 | 79.7±3.61cB | 183.4±3.26bC | 0.90±0.82aA | 78.6±4.16cB | 191.7±3.42bB | 2.43±0.82aA | |
60 | 66.3±1.37dC | 194.9±2.04aC | 1.48±0.60aA | 71.0±2.64dC | 198.4±1.60aB | 2.79±0.60aA |
Each value is mean±SD of triplicate experiments (n=50).
Different small letters indicate significant differences for the different clove oil dose at the same water temperature (p<0.05).
Different capital letters indicate significant differences for the different water temperature at the same clove oil dose (p<0.05).