Table 2.
Fold change in transcripts per million in paired single patient samples (pain:no pain)
| Patient 15 | Patient 29 | Patient 26 | MAPK signalling | TNF-α signalling | TGF-β signalling | TLR signalling | OSM signalling | AHR signalling | SCI | |
|---|---|---|---|---|---|---|---|---|---|---|
| Transcription factors and co-factors | ||||||||||
| JUN | 2.62 | 1.24 | 1.83 | ✓ | ✓ | - | ✓ | - | ✓ | - |
| FOS | 2.60 | 2.00 | 10.40 | ✓ | - | - | ✓ | ✓ | - | ✓ |
| NFKB2 | 2.00 | 1.06 | 0.92 | - | ✓ | - | ✓ | - | - | - |
| RUNX2 | 2.75 | 1.22 | 1.93 | - | - | ✓ | - | - | - | - |
| FOSB | 13.55 | 13.53 | 283.90 | - | - | ✓ | - | - | - | - |
| ATF3 | 7.58 | 1.99 | 1.76 | - | - | ✓ | - | - | - | - |
| JUNB | 3.39 | 1.38 | 4.90 | - | - | ✓ | - | ✓ | ✓ | - |
| EGR1 | 6.28 | 2.47 | 10.19 | - | - | - | - | ✓ | - | ✓ |
| KLF10 | 2.35 | 1.28 | 1.47 | - | - | ✓ | - | - | - | - |
| NR4A1 | 3.21 | 1.01 | 1.76 | ✓ | - | - | - | - | - | ✓ |
| HES1 | 2.41 | 1.17 | 1.36 | - | - | - | - | - | ✓ | - |
| AHR | 2.02 | 1.26 | 1.04 | - | - | - | - | - | ✓ | - |
| BTG2 | 1.23 | 1.11 | 2.79 | - | - | - | - | - | - | ✓ |
| RNA binding proteins | ||||||||||
| ZFP36 | 2.29 | 1.31 | 4.12 | - | - | - | - | - | - | ✓ |
| Cytokine ligands | ||||||||||
| TNF | 3.71 | LE | 19.66 | ✓ | ✓ | - | ✓ | - | ✓ | ✓ |
| IL1B | 8.72 | LE | 9.09 | ✓ | - | - | ✓ | - | ✓ | ✓ |
| IL6 | 9.13 | LE | 4.68 | - | ✓ | - | ✓ | - | - | ✓ |
| IL12A | 3.01 | 1.40 | 1.31 | - | - | - | ✓ | - | ✓ | - |
| CCL2 | 3.04 | 1.00 | 0.93 | - | - | - | - | ✓ | - | ✓ |
| CCL3 | 12.52 | 3.45 | 75.40 | - | - | - | ✓ | - | - | - |
| CCL4 | 23.60 | 1.60 | 3035.20 | - | - | - | ✓ | - | - | - |
| CXCL2 | 1.92 | LE | 2.99 | - | - | - | - | - | - | ✓ |
| OSM | 10.39 | LE | 37.07 | - | - | - | - | ✓ | - | - |
| TGFB1 | 2.13 | 1.09 | 1.81 | ✓ | - | ✓ | - | - | ✓ | ✓ |
| TGFB3 | 4.77 | 1.35 | 1.81 | ✓ | - | ✓ | - | - | - | - |
| GDNF | 8.47 | LE | LE | - | - | - | - | - | - | ✓ |
| Cytokine regulators | ||||||||||
| SOCS3 | 14.41 | 2.84 | 4.64 | - | - | - | ✓ | ✓ | - | - |
| Neurotrophins | ||||||||||
| NGF | 2.30 | 1.04 | 1.39 | ✓ | - | - | - | - | - | - |
Genes involved in important signalling pathways or spinal cord injury, and their fold-change in the three pairs of samples from the same patients with differing pain states show several key transcription factors and cytokines to be upregulated in the pain state. LE = low expression; SCI = spinal cord injury.
✓ = gene set membership.