Figure 3.

Effects of unilateral microinjection of different doses of Sulpiride as a D2-like receptor antagonist, into the dorsal hippocampus (CA1) on intra-VTA orexin-induced CPP (A) In this set of experiments, animals received different doses of (0.25, 1 and 4 µg/0.5 µL DMSO) or DMSO (0.5 μL/side) as a vehicle in the CA1, 5 min prior to intra-VTA injection of orexin A (100 ng/0.3 µL saline) during the 3-day conditioning period and the conditioning scores were measured in post-conditioning day. (B) Mean locomotor activity of all the groups in this set of experiment during 10-min period on post-test day. The anatomical control group (n = 6) received the highest dose of Sulpiride (4 µg/0.5 µL DMSO), 5 min prior to orexin-induced conditioned place preference. All data are expressed as mean ± SEM for 6-7 rats. *P <0.05, ***P < 0.001 different from the vehicle-control group (white bar). †††P < 0.001 different from the control group (black bar)