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. 2019 Winter;18(1):241–253.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of Halobacterium salinarum IBRC M10715 supernatant metabolite on stemness properties of prostate cancer cell lines. (A) 106 cells of treated with 0.5 mg/mL concentration of Halobacterium salinarum SM per T25 flask were subjected to sphere formation in no-adherent conditions. Untreated cells were used as control group. (B) The percentage of spheres pre and post treatment was calculated by dividing the number of spheroids to the number of seeding number of seeding number ´ 100. (C) Size of spheres in pre and post 48 h treatment of PC3 and DU145. (D) The relative expression of SOX2 gene (as pluripotency marker) was assessed by quantitative real-time PCR. The GAPDH was used for normalizing of data. Bars indicated Mean± SD of at least 3 biological replicates, (***p < 0.001).