Figure 4.

Water restriction significantly increases protein abundance of AQP2 and NKCC2 in the non‐SCD and SCD mouse outer medullas (A and B) and of AQP2 and AQP2‐S256‐P in the non‐SCD and SCD mouse inner medullas (E and F), and these effects are not significantly different between these two strains. Water restriction decreases UTA1 protein abundance in the non‐SCD inner medulla, whereas it has no significant effect in the SCD one (G). Water restriction has no significant effect on protein abundance of ENaC‐α, β or γ in the outer medulla (C and D) or AQP1 protein level (H) in the inner medulla of non‐SCD or SCD group. Mice were treated in the same way as in Figure 1 (*P < 0.05 vs. the corresponding water replete group, n = 6 in each group).