Table 1.
Experimental design and animal numbers used per group. None of sham operated animals died in this study. Average mortality was 8.8% in the experimental animals. No significant difference between treated and untreated subgroups was observed in the mortality. BWC, brain water content; IHC, immunohistochemistry; MPO, myeloperoxidase; ICH + HG, hyperglycemic intracerebral hemorrhage; Vehicle, 0.9% sterile NaCl; siRNA, small interfering ribonucleic acid; Coumermycin A1, an agonist of JAK2.
| Groups | Neuro test BWC |
Western blot | IHC MPO | Mortality |
Sum | ||
|---|---|---|---|---|---|---|---|
| 24 h | 72 h | 24 h | 72 h | ||||
| Time course study (n = 6; 9 per group): Sham | – | 6 | 3 | – | 9 | ||
| ICH + HG (6, 12, 24, 48, 72 h) | – | 30 (5) | 3 | 5 (13%) | 38 | ||
| Outcome and Mechanism study: | |||||||
| Sham | 6 | 6 | – | – | – | – | 12 (6 + 6) |
| ICH + HG + Vehicle | 6 (1) | 6 (2) | 6 (1) | 3 | 2 (12%) | 2 (25%) | 25 (17 + 8) |
| ICH + HG + rOPN | 18 (1) | 6 (1) | 6 | 3 | 1 (4%) | 1 (14%) | 35 (28 + 7) |
| ICH + HG + Scramble siRNA | 6 | 3 | – | 9 | |||
| ICH + HG + Integrin-β1 siRNA | 6 | 3 | – | 9 | |||
| ICH + HG + rOPN + Coumermycin A1 | 6 (1) | 3 (1) | 2 (18%) | 11 | |||
| Total | 30 | 18 | 66 | 21 | 13 (8.8%) | 148 | |