The effects of 2 h restraint stress on AEA, 2‐AG, PEA and OEA content in the hypothalamus (A–D), serum (E–H), jejunum (I–L) and duodenum (M–P). Acute restraint (n = 10) resulted in decreased AEA content in the hypothalamus (A) and in serum (E) compared to unstressed homecage animals (HC; n = 10). However, no other changes in endocannabinoids or levels of PEA or OEA were observed in these regions. In the small intestine, 2‐AG content was selectively decreased in the jejunum following stress, but not in duodenum. Intestinal content of AEA, PEA and OEA in these regions were unaffected by stress. Individual results are shown with mean ± SEM indicated by the bars. *
P < 0.05, significantly different from homecage unstressed group.