Fig. 3.

Analysis of SR-BI down-regulation. A, HUVECs were incubated for 24 h with 20 nM rapamycin (R) and/or 10 μM lovastatin (L). Cells were lysed and quantitative real time PCR was performed (n = 3). B, after preincubation with rapamycin and/or lovastatin, cells were fixed, filipin staining was performed, and cells were imaged using a fluorescence microscope. C, HUVECs were incubated for 24 h with 20 nM rapamycin and/or 10 μM troglitazone or 10 μM pioglitazone. Cells were lysed and Western Blot analysis was performed. *p < 0.005.