Fig. 6.

Influence of SR-BI knockdown on HDL-stimulated eNOS activation and cell migration. A, after lentiviral knockdown of SR-BI HUVECs were incubated with or without 100 μg/ml HDL for 30 min. Cells were lysed and analyzed by Western Blot (n = 3). A representative Western Blot is shown. B, HUVEC monolayers were scratched and migration into the wounded area was assessed after 6 and 24 h in the presence of 20 nM rapamycin and/or 100 μg/ml HDL. Cell migration after 24 h was quantified. A representative image is shown. Results are calculated as % of the initial scratch area; results are expressed as % of control (n = 3). *p < 0.005.